Assisted Hatching for In Vitro Fertilization-Embryo Transfer: An Update

Assisted Hatching (AH) is a technique performed after In Vitro Fertilization (IVF) and involves the artificial thinning or opening of the Zona Pellucida (ZP) prior to Embryo Transfer (ET) as an attempt to improve the probability of embryo implantation. AH can be performed by embryologists via mechanical, chemical, or laser-assisted means. A few studies suggest that a larger size of ZP opening/thinning as well as a site near the ICM may be associated with a greater probability for complete hatching. It is not recommended to apply AH to all IVF cycles universally. Subgroups of patients that may benefit from AH include those with prior implantation failure, those undergoing Frozen-Thawed Embryo Transfer (FET) cycles, and women who are 38 years of age or older. IVF programs should identify subgroups of women within their patient population who may benefit clinically from AH.